中捷四方-大丽轮枝菌激活蛋白VdAL（维大利）的创制与功能研究（50页）.pdf

1、大丽轮枝菌激活蛋白VdAL(维大利)的创制与功能研究 Development and Function study of activator protein VdAL from Verticillium dahliaJunsheng Qi；Zhizhong GongChina Agricultural University,College of Biological SciencesState Key Laboratory of Plant Physiology and B； Yinzhong Cui；Haitao QianPherobio Technology Co.,L http:/ Co

2、ntents lWhat is VdAL（来源及性质）lHow VdAL was produced（生产方法）lHow to use VdAL and its optimum concentrations（推荐用法）lVdAL induces resistance to disease and abiotic stresses（抗病）lVdAL promotes crop growth and increase yield（增产）lVdAL promotes fruit maturation and improve quality（改善品质）lThe possible mechanism of

3、 VdAL（作用机理）What is VdAL(维大利)?CottonStraw berry Egg plant Sun flowerVdAL:A secreted protein from Verticillium dahlia(大丽轮枝菌),which is a fungal pathogen that causes vascular wilt diseases in many plant species,such as cotton,straw berry,egg plant,sun flower.Qi Junsheng Gong ZhizhongVdAL is an ASPF2-lik

4、e secreted protein with 297 AAYTK12 pSUC2YTK12 pSUC2-MSP-MSP pSUC2-VdAL pSUC2-VdALTTCTTCYPRAAYPRAACMDCMD-W-WYPDA YPDA VdAL:Verticillium dahliae Asp-f2 LikeVdAL is a zinc ion binding protein（锌离子结合蛋白）WT line 2 line 3WT line 2 line 3Line 2 line 3 WTVdALUBQDisease Index(%)020406080100*WT line 2 line 3AC

5、BDExpression of VdAL increases disease resistance to Verticillium in Arabidopsis （拟南芥表现抗黄萎病）VdALLine 2VdALLine 3WTVdAL treatment induce the resistance to Verticillium wilting （诱导棉花对黄萎病抗性）CK72 h144 hThe cotton leaves were first treated with VdAL,and then the petioles were soaked in the Verticillium s

6、olution for different times.Plant vaccine？植物疫苗？treatmentCKtreatment VdAL patents：3 Chinese national invention patents 3项国内专利 共申请共申请13个国家专利保护。澳大利亚、哈萨克斯坦、俄罗斯已授权个国家专利保护。澳大利亚、哈萨克斯坦、俄罗斯已授权VdAL patents：2 PCTs applied in:Australia、Brazil、Canada、EU、India、Russia、South Africa、America、Chile、Indonesia、Kazakhsta