郑海学-非洲猪瘟感染细胞的嗜性及其应答机制.pdf

1、郑海学 Haixue Zheng中国农业科学院兰州兽医研究所Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,China 2024年4月非洲猪瘟病毒猪体内感染靶细胞及其调控复制的机制 The Infectious Cell Tropism and Its Regulatory Replication Mechanism of ASFV In Vivo一带一路国际生猪产业大会非洲猪瘟严重危害养猪业African swine fever(ASF)seriously threa

2、tens the pig industry2018年，非洲猪瘟传入我国，给我国生猪产业造成了前所未有的经济损失。非洲猪瘟在世界范围内流行已逾百年，至今没有商品化疫苗可用，这是全球生猪行业的痛点和难点。非洲猪瘟病毒能够导致免疫器官病变和功能衰竭而死，这是防治的难题。数据来自WOAH 截止2022/10/28一带一路国际生猪产业大会ASFV是如何在动物体内快速繁殖并引起发病？How ASFV can replicate rapidly and cause acute disease in animals after infection?免疫系统三道防线炎炎症症Inflammation天天然然免免疫

3、疫Innate immunity获获得得性性免免疫疫Adaptive immunity清清除除病病毒毒Virus clearance康复或死亡Recovered or Died淋巴细胞分化一带一路国际生猪产业大会我们前期研究发现 Our findingsASFV引起天然免疫抑制的机制The mechanisms of suppression of innate immune response by ASFV？1.ASFV调节RIG-I介导的天然免疫 Regulation of RIG-I-mediated innate immunity by ASFV2.ASFV调节cGAS-STING介导的

4、天然免疫 Regulation of cGAS-STING-mediated innate immunity by ASFV 3.ASFV调节II型信号通路 Regulation of type II signaling pathway by ASFV一带一路国际生猪产业大会I267L VirusMitoViral RNAVISATBK1IRF3PPPIFN-b b/a aISREIRF3IRF3RIG-IMITARipletK63-linked UbPloS Pathogens.(2022,28,18(1):e1010270)兰兰兽兽研研、武武汉汉病病毒毒所所I267L与Riplet有相互作

5、用 I267l interacts with Riplet I267L通过Riplet抑制RIG-I的K63泛素化从而抑制干扰素的产生 I267l inhibits production of interferon by inhibiting K63 ubiquitination of RIG-I.I267L是ASFV的毒力因子之一I267l is one of the virulence factors of ASFVASFV 调控RIG-I介导的天然免疫的机制I267L inhibits ASFV-induced type-I interferon production by target

6、ing RNA Polymerase III-RIG-I signaling一带一路国际生猪产业大会MGF505-7R interacted with STINGMGF505-7R inhibited IFN production through increasing the expression of autophagy-related proteinMGF505-7R is a newly identified virulence factor.MGF505-7R与STING有相互作用MGF505-7R通过上调自噬相关蛋白ULK1的表达降解STING从而抑制干扰素的产生MGF505-7R是