CONF-4-Lionel-Pineau.pdf

1、Comparison of endoscope sampling and culturing methodsLionel PINEAUEurofins Hospital HygieneDisclosure of Conflicts of Interest:Eurofins Biotech Germandereports having been consulted and having received financialsupport from medical device manufacturers to performed studieson the efficacy of medical

2、 devices.Introduction2Endoscope contamination rateMicrobial contaminationInfectious risk?What is the microbiological quality of our endoscopes?Is there a risk for the patients?IntroductionIn 2021,in France,13%of endoscopes are at the action level and 8.1%are at the alert level,meaning that the conta

3、mination level of 21.1%of endoscopes exceeds what has been defined as a maximum acceptable value.Pineau Lionel.Endoscope reprocessing:Retrospective analysis of 90,311 samples.Endosc In t Open 2023;11:E247E257010203040506070809010020002005201020152020%of non-compliant endoscopesSaviuc2015Minebois2016

4、Studies published in the literature indicate that the non-compliance rate of ready to use endoscopes varies from 0.4%to 49.0%Chiu2012Gillespie 2008Rauwers2018Bader 2001Saliou2010Okamoto2022Chomel2009Mark2020Moses 2003Alfa 2002Osborne 2007IntroductionBader L et al,2002 In 2 test periods,endoscopes re

5、ady for use were found contaminated at high rates:Period 1:49%of 152 endoscopes;Period 2:39%of 154 endoscopes).0,9%NaClSuction(20 ml)Biopsy(20 ml)Total viable countPlate-Count agar plates(20C/37C for 48 hours)Sampling and culturing method1ml10 mlPathogensMembrane filtration Brain-Heart-Infusion agar

6、(37C for 48 h)Swab on 5%Blood-Columbia agar and MacConkey agar(37C for 48 h)Air/water(20 ml)Water bottle Distilled water,tap water or sterile water60 mlGillespie et al,2007Blood agar&MacConkey(35C/48 hours+28C for 3 days)2 x 0,1 ml10 ml10 ml in each channelSterile water40 ml1 ml9 ml withdrawn40 CFU