1、Biacore application guidesAntibody screening with Biacore systems2Antibody screening with Biacore systemsIn the search for therapeutic or analytical antibodies the selection of appropriate kinetics/affinity,specificity and biophysical properties is essential.Antibody screening aims to identify cell

2、clones that produce antibodies appropriate for the purpose,by challenging the antibodies with known antigen.Sample matrices are often complex,e.g.hybridoma culture supernatants or phage display preparations.Obtaining kinetic information early in the screening process is often a significant advantage

3、.Information that is generally sought includes:Specificity which clones produce antibodies with the desired specificity?Binding characteristics which clones produce antibodies with suitable kinetic and/or affinity properties?Expression level which clones produce sufficient amounts of antibodies?Scre

4、ening can be performed on full size antibodies or parts thereof,such as Fab fragments or scFvs.This Application guide deals with screening of complete monoclonal IgG antibodies.Assay conditions and prerequisites might differ for other antibody types and fragments.3Assay formatAntibody screening can

5、be run using two different assay formats:Format DescriptionAntibody as ligand Antigen as analyteSamples to be screened are injected over a generic antibody-capturing surface.Antigen is then injected as analyte.The surface is regenerated by removing captured antibody and bound antigen.Antigen as liga

6、nd Antibody as analyteAntigen is immobilized or captured on the surface.Samples to be screened are then injected as analyte.The surface is regenerated by removing the bound antibody.(If reversible antigen capture is used,the surface may be regenerated by removing captured antigen and bound antibody)